Pipeline for the identification of extrachromosomal circular DNA (ecDNA) from Circle-seq, WGS, and ATAC-seq data that were generated from cancer and other eukaryotic cells.
v1.0.3 - [2023-06-05] - Golden Wombat
- Licence, contact, source information for AmpliconArchitect and PrepareAA python scripts - Recommendation by @SPPearce
- documentation about absolute path needed of AmpliconArchitect data repository - Recommendation by @SPPearce
- ampliconclassifier stub run tests
- new version of circdna metromap with updated colors
- note that ATAC-seq should be used in caution with the pipeline.
- build docker container for prepareaa -> Needs to be built first and will be included in the next release - Thanks to @ewels
- nf-core template update 2.8 - Thanks to the nf-core team
- newest versions of
AmpliconArchitect- Recommendation and help from @jluebeck
- Circle_finder bug with bash sort command wanting to write into /tmp/ directory and not into work directory
- Usage.md updated to new paths and addition of nf-core modules
- Local python scripts not included in the pipeline
- Local versions of nf-core modules
Thanks to the reviewers @jluebeck and @SPPearce for providing relevant feedback for improvements and clarity of nf-core/circdna. Also thanks to @ewels for the comprehensive help in building the docker image for
PrepareAA for future releases.
v1.0.2 - [2023-03-09] - Bronze Pigeon
- ampliconclassifier/makeinput module added -> Generates the input file used for ampliconclassifier functions
- ampliconclassifier/makeresultstable added -> Generates results table from AmpliconArchitect and AmpliconClassifier
- CNN Reference File For AmpliconArchitect
- mm10 option for AmpliconArchitect
- stub runs for AmpliconArchitect processes
- New module versions
- nf-core template 2.7.2
- Fixed ZeroDivisionError by Circle-Map
- Fixed keep_duplicates and skip_markduplicates parameter bug
- AmpliconArchitect Summary Process was deprecated
v1.0.1 - [2022-06-22] - Black Bronze Viper
In this Release, a bug was fixed in the nextflow.config and the documentation was updated.
- Documentation Updates
- Fixed Bug with pipeline version in nextflow.config
- Fixed Circle-Map Realign bug in which only one sample is processed
- Samtools FAIDX
nf-core/circdna is a bioinformatics analysis pipeline for the identification of circular DNAs in eukaryotic cells. The pipeline is able to process WGS, ATAC-seq data or Circle-Seq data to give insights into the circular DNA landscape in your samples.
In total, the user can choose between 5 different branches inside the pipeline, depending on the biological question and the input data set. In these branches, specific software is used that is built for either the identification of amplified circular DNAs, the detection of putative circular DNA junctions, or the de novo assembly and mapping of circular DNAs.