Define where the pipeline should find input data and save output data.

Path to comma-separated file containing information about the samples in the experiment.

required
type: string
pattern: ^\S+\.csv$

The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.

required
type: string

Email address for completion summary.

type: string
pattern: ^([a-zA-Z0-9_\-\.]+)@([a-zA-Z0-9_\-\.]+)\.([a-zA-Z]{2,5})$

MultiQC report title. Printed as page header, used for filename if not otherwise specified.

type: string

Reference genome related files and options required for the workflow.

Path to FASTA reference genome file.

required
type: string
pattern: ^\S+\.fn?a(sta)?(\.gz)?$

Path to FASTA index (.fai) file for the reference genome.

type: string
pattern: ^\S+\.fn?a(sta)?(\.gz)?\.fai$

Path to sequence dictionary (.dict) for the reference genome.

type: string
pattern: ^\S+\.dict$

Path to directory containing the BWA-MEM2 index for the reference genome.

type: string

Path to directory containing the intervals in bed format.

type: string

Number of interval files to split the reference genome into for parallel processing.

type: integer

Maximum number of intervals per interval file for parallel processing.

type: integer

Options for providing an existing known variant set or generating one via bootstrapping.

Path to known variant set vcf file.

type: string,null
pattern: ^\S+\.vcf\.gz$

Path to known variant set tabix index tbi file.

type: string,null
pattern: ^\S+\.vcf\.gz\.tbi$

Number of bootstrapping rounds to perform when generating a variant set.

type: integer,null

List of scaffolds (e.g., chromosome or contig names) to include using vcftools. Can be passed as single string, array of strings, path to text file (including .bed). Leave empty to choose all by default.

type: string,array,null

List of scaffolds (e.g., chromosome or contig names) to exclude using vcftools. Can be passed as single string, array of strings, path to text file (including .bed). Leave empty to disable exclusion.

type: string,array,null

Options for pipeline stages to run.

Whether to perform hard filtering of variants.

type: boolean

Whether to skip Base Quality Score Recalibration (BQSR) on the aligned reads.

type: boolean

Whether to skip the variant calling stage to identify variants from the aligned reads.

type: boolean

Whether to skip the relatedness estimation stage.

type: boolean

Parameters used to describe centralised config profiles. These should not be edited.

Git commit id for Institutional configs.

hidden
type: string
default: master

Base directory for Institutional configs.

hidden
type: string
default: https://raw.githubusercontent.com/nf-core/configs/master

Institutional config name.

hidden
type: string

Institutional config description.

hidden
type: string

Institutional config contact information.

hidden
type: string

Institutional config URL link.

hidden
type: string

Less common options for the pipeline, typically set in a config file.

Display version and exit.

hidden
type: boolean

Method used to save pipeline results to output directory.

hidden
type: string

Email address for completion summary, only when pipeline fails.

hidden
type: string
pattern: ^([a-zA-Z0-9_\-\.]+)@([a-zA-Z0-9_\-\.]+)\.([a-zA-Z]{2,5})$

Send plain-text email instead of HTML.

hidden
type: boolean

File size limit when attaching MultiQC reports to summary emails.

hidden
type: string
default: 25.MB
pattern: ^\d+(\.\d+)?\.?\s*(K|M|G|T)?B$

Do not use coloured log outputs.

hidden
type: boolean

Incoming hook URL for messaging service

hidden
type: string

Custom config file to supply to MultiQC.

hidden
type: string

Custom logo file to supply to MultiQC. File name must also be set in the MultiQC config file

hidden
type: string

Custom MultiQC yaml file containing HTML including a methods description.

type: string

Boolean whether to validate parameters against the schema at runtime

hidden
type: boolean
default: true

Base URL or local path to location of pipeline test dataset files

hidden
type: string
default: https://raw.githubusercontent.com/nf-core/test-datasets/

Suffix to add to the trace report filename. Default is the date and time in the format yyyy-MM-dd_HH-mm-ss.

hidden
type: string

Display the help message.

type: boolean,string

Display the full detailed help message.

type: boolean

Display hidden parameters in the help message (only works when —help or —help_full are provided).

type: boolean