This pipeline is based on the HiC-Pro workflow. It was designed to process Hi-C data from raw fastq files (paired-end Illumina data) to normalized contact maps. The current version supports most protocols, including digestion protocols as well as protocols that do not require restriction enzymes such as DNase Hi-C. In practice, this workflow was successfully applied to many data-sets including dilution Hi-C, in situ Hi-C, DNase Hi-C, Micro-C, capture-C, capture Hi-C or HiChip data.

The pipeline is built using Nextflow, a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It comes with docker / singularity containers making installation trivial and results highly reproducible.

Pipeline summary

  1. Mapping using a two steps strategy to rescue reads spanning the ligation sites (bowtie2)
  2. Detection of valid interaction products
  3. Duplicates removal
  4. Create genome-wide contact maps at various resolution
  5. Contact maps normalization using the ICE algorithm (iced)
  6. Quality controls and report (MultiQC)
  7. Addition export for visualisation and downstream analysis (cooler)


The nf-core/hic pipeline comes with documentation about the pipeline, found in the docs/ directory:

  1. Installation
  2. Pipeline configuration
  3. Running the pipeline
  4. Output and how to interpret the results
  5. Troubleshooting


nf-core/hic was originally written by Nicolas Servant.