Description

Trim sequencing adapters and collapse overlapping reads

Input

Name (Type)
Description
Pattern

meta (map)

Groovy Map containing sample information
e.g. [ id:‘test’, single_end

]

reads (file)

List of input FastQ files of size 1 and 2 for single-end and paired-end data,
respectively.

*.{fq,fastq,fq.gz,fastq.gz}

adapterlist (file)

Optional text file containing list of adapters to look for for removal with one adapter per line. Otherwise will look for default adapters (see AdapterRemoval man page), or can be modified to remove user-specified adapters via ext.args.

Output

Name (Type)
Description
Pattern

singles_truncated (file)

Adapter trimmed FastQ files of either single-end reads, or singleton
’orphaned’ reads from merging of paired-end data (i.e., one of the pair
was lost due to filtering thresholds).

*.truncated.fastq.gz

discarded (file)

Adapter trimmed FastQ files of reads that did not pass filtering
thresholds.

*.discarded.fastq.gz

paired_truncated (file)

Adapter trimmed R{1,2} FastQ files of paired-end reads that did not merge
with their respective R{1,2} pair due to long templates. The respective pair
is stored in ‘pair{1,2}_truncated’.

*.pair{1,2}.truncated.fastq.gz

collapsed (file)

Collapsed FastQ of paired-end reads that successfully merged with their
respective R1 pair but were not trimmed.

*.collapsed.fastq.gz

collapsed_truncated (file)

Collapsed FastQ of paired-end reads that successfully merged with their
respective R1 pair and were trimmed of adapter due to sufficient overlap.

*.collapsed.truncated.fastq.gz

paired_interleaved (file)

Write paired-end reads to a single file, interleaving mate 1 and mate 2 reads

*.paired.fastq.gz

settings (file)

AdapterRemoval log file

*.settings

versions (file)

File containing software versions

versions.yml

Tools

adapterremoval
GPL v3

The AdapterRemoval v2 tool for merging and clipping reads.