Description

Calculate read duplication rate

Input

Name (Type)
Description
Pattern

meta (map)

Groovy Map containing sample information
e.g. [ id:‘test’, single_end

]

bam (file)

the alignment in bam format

*.{bam}

bed (file)

a bed file for the reference gene model

*.{bed}

Output

Name (Type)
Description
Pattern

seq_xls (file)

Read duplication rate determined from mapping position of read

*seq.DupRate.xls

pos_xls (file)

Read duplication rate determined from sequence of read

*pos.DupRate.xls

pdf (file)

plot of duplication rate

*.pdf

rscript (file)

script to reproduce the plot

*.R

versions (file)

File containing software versions

versions.yml

Tools

rseqc
GPL-3.0-or-later

RSeQC package provides a number of useful modules that can comprehensively evaluate high throughput sequence data especially RNA-seq data.