Description

Align reads to a reference genome using bowtie2 then sort with samtools

Input

Name (Type)
Description
Pattern

meta (map)

Groovy Map containing sample information
e.g. [ id:‘test’, single_end

]

ch_reads (file)

List of input FastQ files of size 1 and 2 for single-end and paired-end data,
respectively.

ch_index (file)

Bowtie2 genome index files

*.ebwt

save_unaligned (boolean)

Save reads that do not map to the reference (true) or discard them (false)
(default: false)

sort_bam (boolean)

Use samtools sort (true) or samtools view (false)

ch_fasta (file)

Reference fasta file

*.{fasta,fa}

Output

Name (Type)
Description
Pattern

bam (file)

Output BAM file containing read alignments

*.{bam}

versions (file)

File containing software versions

versions.yml

fastq (file)

Unaligned FastQ files

*.fastq.gz

log (file)

Alignment log

*.log