Description

Align reads to a reference genome using bowtie2 then sort with samtools

Input

name:type
description
pattern

meta :map

Groovy Map containing sample information
e.g. [ id:‘test’, single_end

]

ch_reads :file

List of input FastQ files of size 1 and 2 for single-end and paired-end data,
respectively.

ch_index :file

Bowtie2 genome index files

*.ebwt

save_unaligned :boolean

Save reads that do not map to the reference (true) or discard them (false)
(default: false)

sort_bam :boolean

Use samtools sort (true) or samtools view (false)

ch_fasta :file

Reference fasta file

*.{fasta,fa}

Output

name:type
description
pattern

bam :file

Output BAM file containing read alignments

*.{bam}

versions :file

File containing software versions

versions.yml

fastq :file

Unaligned FastQ files

*.fastq.gz

log :file

Alignment log

*.log