subworkflows/fastq_align_star

Align reads to a reference genome using bowtie2 then sort with samtools

alignfastagenomereference

https://github.com/nf-core/modules/[...]/subworkflows/nf-core/fastq_align_star

Description

Align reads to a reference genome using bowtie2 then sort with samtools

Input

Name (Type)

Description

Pattern

`ch_reads`

List of input FastQ files of size 1 and 2 for single-end and paired-end data,
respectively.
Structure: [ val(meta), [ path(reads) ] ]

`ch_index` (directory)

STAR genome index

star

`ch_gtf` (file)

GTF file used to set the splice junctions with the —sjdbGTFfile flag

*.gtf

`val_star_ignore_sjdbgtf` (boolean)

If true the —sjdbGTFfile flag is set

true|false

`val_seq_platform` (string)

Sequencing platform to be added to the bam header using the —outSAMattrRGline flag

`val_seq_center` (string)

Sequencing center to be added to the bam header using the —outSAMattrRGline flag

`ch_fasta` (file)

Reference genome fasta file

*.{fasta,fa,fna}

Output

Name (Type)

Description

Pattern

`orig_bam`

Output BAM file containing read alignments
Structure: [ val(meta), path(bam) ]

`log_final`

STAR final log file
Structure: [ val(meta), path(log_final) ]

`log_out`

STAR log out file
Structure: [ val(meta), path(log_out) ]

`log_progress`

STAR log progress file
Structure: [ val(meta), path(log_progress) ]

`bam_sorted`

Sorted BAM file of read alignments (optional)
Structure: [ val(meta), path(bam) ]

`bam_transcript`

Output BAM file of transcriptome alignment (optional)
Structure: [ val(meta), path(bam) ]

`fastq`

Unmapped FastQ files (optional)
Structure: [ val(meta), path(fastq) ]

`tab`

STAR output tab file(s) (optional)
Structure: [ val(meta), path(tab) ]

`stats`

File containing samtools stats output
Structure: [ val(meta), path(stats) ]

`bam`

BAM file ordered by samtools
Structure: [ val(meta), path(bam) ]

`bai`

BAI index of the ordered BAM file
Structure: [ val(meta), path(bai) ]

`flagstat`

File containing samtools flagstat output
Structure: [ val(meta), path(flagstat) ]

`idxstats`

File containing samtools idxstats output
Structure: [ val(meta), path(idxstats) ]

`versions` (file)

File containing software versions

versions.yml

subworkflows/fastq_align_star

Description

Input

`ch_reads`

`ch_index` (directory)

`ch_gtf` (file)

`val_star_ignore_sjdbgtf` (boolean)

`val_seq_platform` (string)

`val_seq_center` (string)

`ch_fasta` (file)

Output

`orig_bam`

`log_final`

`log_out`

`log_progress`

`bam_sorted`

`bam_transcript`

`fastq`

`tab`

`stats`

`bam`

`bai`

`flagstat`

`idxstats`

`versions` (file)

included in

author

get in touch

subworkflows/fastq_align_star

Description

Input

ch_reads

ch_index (directory)

ch_gtf (file)

val_star_ignore_sjdbgtf (boolean)

val_seq_platform (string)

val_seq_center (string)

ch_fasta (file)

Output

orig_bam

log_final

log_out

log_progress

bam_sorted

bam_transcript

fastq

tab

stats

bam

bai

flagstat

idxstats

versions (file)

included in

author

get in touch

`ch_reads`

`ch_index` (directory)

`ch_gtf` (file)

`val_star_ignore_sjdbgtf` (boolean)

`val_seq_platform` (string)

`val_seq_center` (string)

`ch_fasta` (file)

`orig_bam`

`log_final`

`log_out`

`log_progress`

`bam_sorted`

`bam_transcript`

`fastq`

`tab`

`stats`

`bam`

`bai`

`flagstat`

`idxstats`

`versions` (file)