nf-core/clipseq
CLIP sequencing analysis pipeline for QC, pre-mapping, genome mapping, UMI deduplication, and multiple peak-calling options.
clipclip-seqpeak-callingrna-rbp-interactions
This pipeline uses DSL1. It will not work with Nextflow versions after
Launch version 1.0.0 https://github.com/nf-core/clipseq 22.10.6
.
Learn more.
Version history
Initial release of nf-core/clipseq, created with the nf-core template.
Pipeline summary
- Adapter and quality trimming (
Cutadapt
) - Pre-mapping to e.g. rRNA and tRNA sequences (
Bowtie 2
) - Genome mapping (
STAR
) - UMI-based deduplication (
UMI-tools
) - Crosslink identification (
BEDTools
) - Bedgraph coverage track generation (
BEDTools
) - Peak calling (multiple options):
iCount
Paraclu
PureCLIP
Piranha
- Motif detection (
DREME
) - Quality control:
- Sequencing quality control (
FastQC
) - Library complexity (
Preseq
) - Regional distribution (
RSeQC
)
- Sequencing quality control (
- Overall pipeline run and QC summaries and peak calling comparisons (
MultiQC
)