What’s Changed

• Better support for custom chemistries by @grst in #273
• Important! Template update for nf-core/tools v2.11.1 by @nf-core-bot in #279
• Adding cellranger-arc (multiome scRNA-seq + scATAC support) by @heylf in #274
• increase time limits by @grst in #281
• Fix time limits by @grst in #284

Full Changelog: https://github.com/nf-core/scrnaseq/blob/dev/CHANGELOG.md#v250---2024-01-02

What’s Changed

• fix cellranger filename check by @grst in #261
• Fix 263 by @grst in #267
• Important! Template update for nf-core/tools v2.10 by @nf-core-bot in #269
• Release v2.4.1 by @grst in #268

Full Changelog: 2.4.0…2.4.1

What’s Changed

• Fix typo causing empty version imformation for mtx_conversion subworkflow (#254)
• Add singularity.registry = 'quay.io' and bump NF version to 23.04.0 (#237)
• Fixed issue with file collisions while using cellranger (#232)
• Fix issue where multiqc inputs tried to access objects that did not exist (#239)
• Removed public_aws_ecr profile (#242)
• Include cellranger in MultiQC report (#244)
• Nf-core template update to v2.9 (#245)
• Update cellranger and fastqc module (#246). The updated cellranger module now automatically renames input FASTQ files to match the expected naming conventions.

New Contributors

• @shinthor made their first contribution in #254

This release is focused simply on ensuring as many containers as possible are hosted on quay.io.

What’s Changed

• Move containers for pipeline to quay.io by @drpatelh in #233
• Dev -> Master for 2.3.2 release by @drpatelh in #234

Full Changelog: 2.3.1…2.3.2

What’s Changed

• Important! Template update for nf-core/tools v2.8 by @nf-core-bot in #221
• Public aws ecr profile by @adamrtalbot in #225
• Fix code comment by @stan0610 in #224
• Update docs for release 2.3.1 by @adamrtalbot in #227
• Remove globs from ecr profile by @adamrtalbot in #228
• Release candidate v2.3.1 by @adamrtalbot in #226

New Contributors

• @adamrtalbot made their first contribution in #225
• @stan0610 made their first contribution in #224

Full Changelog: 2.3.0…2.3.1

Enhancements & fixes

• Fix problem on samplesheet check related to amount of columns ([#211])
• 211 samplesheet error in v220 when more than 3 fields are present by @fmalmeida in #216
• Remove unused —bustools_correct and —skip_bustools and fix bugs in schema defaults by @drpatelh in #219
• Fixed bug in starsolo output cardinality by @robsyme in #220

Full Changelog: 2.2.0…2.3.0

What’s Changed

See CHANGELOG.md

New Contributors

• @kafkasl made their first contribution in #163
• @ameynert made their first contribution in #164
• @Vivian-chen16 made their first contribution in #168
• @TomKellyGenetics made their first contribution in #185
• @azedine-healx made their first contribution in #202

v2.1.0 - 2022-10-06 “Green Mercury Siberian Husky”

• Alevin workflow updated to use Alevin-Fry via simpleaf - thanks to @rob-p for supporting this and @fmalmeida implementing the support

Fixes

• Fixed Kallistobustools workflow #123 by upgrading to nf-core/modules module
• Fixed matrix conversion error when running STAR with —soloFeatures GeneFull #135
• Fixed seurat matrix conversion error when running with conda profile #136
• Fixed Kallistobustools module #116. By updating nf-core module and making sure conversion modules take into account the different outputs produced by kallisto standard and non-standard workflows.
• Updated pipeline template to nf-core/tools 2.6

What’s Changed (detailed version)

• Bump version dev by @apeltzer in #114
• Add mimetype to input parameter in schema by @drpatelh in #118
• Addition of MTX_TO_H5AD conversion modules by @fmalmeida in #117
• Dev - Adding FastQC to the pipeline, with integration into MultiQC by @heylf in #121
• Add seurat conversion by @fmalmeida in #125
• KBTools update to nf-core module by @apeltzer in #126
• fixing paths after kallisto update by @fmalmeida in #127
• PR: kallisto fix by @fmalmeida in #131
• Fix README shields / zenodo info by @mribeirodantas in #132
• fix: STAR mtx conversion when using GeneFull by @RHReynolds in #135
• fix(mtx_to_seurat): could not find ReadMtx using conda profile by @RHReynolds in #136
• Parse kallisto non-standard (spliced and unspliced) outputs by @fmalmeida in #133
• FIX: add “else if” instead of if-if by @fmalmeida in #140
• Important! Template update for nf-core/tools v2.5.1 by @nf-core-bot in #143
• Add def for print_error function by @vjmarteau in #145
• Repair inconsistency by @Khajidu in #148
• repair inconsistency by @Khajidu in #149
• Important! Template update for nf-core/tools v2.6 by @nf-core-bot in #151
• Switch from alevin to alevin-fry (with simpleaf) by @fmalmeida in #139
• Enable iGenomes by @robsyme in #144
• Release PR 2.1.0 by @apeltzer in #154

New Contributors

• @drpatelh made their first contribution in #118
• @heylf made their first contribution in #121
• @mribeirodantas made their first contribution in #132
• @RHReynolds made their first contribution in #135
• @vjmarteau made their first contribution in #145
• @Khajidu made their first contribution in #148
• @robsyme made their first contribution in #144

Full Changelog: 2.0.0…2.1.0

v2.0.0 - 2022-06-17 “Gray Nickel Beagle”

• Pipeline ported to dsl2
• Template update with latest nf-core/tools v2.1
• Added cellranger v.7.0.0 subworkflow
• Added full size tests

Fixes

• Make sure pipeline runs on multiple samples #77
• Fix issue where STARsolo always uses 10XV2 chemistry #60

What’s Changed

• DSL2 version with working alevin workflow by @KevinMenden in #55
• Remove social preview image to use GitHub OpenGraph by @maxulysse in #57
• Added kallistobustools by @KevinMenden in #56
• Update to new file structure by @KevinMenden in #62
• Merge template update v2.1 by @ggabernet in #74
• DSL2 conversion by @ggabernet in #73
• Fix kallistobus didn’t run with multiple samples by @grst in #77
• Fix alevinqc by @grst in #79
• Important! Template update for nf-core/tools v2.2 by @nf-core-bot in #76
• Update README.md by @medulka in #59
• Refactor workflows into subworkflows by @grst in #87
• Integrate cellranger workflow by @grst in #90
• Important! Template update for nf-core/tools v2.4 by @nf-core-bot in #101
• Fix Lint & Update latest modules by @apeltzer in #102
• Add AWS Full Size Tests by @apeltzer in #103
• Updating docs by @fmalmeida in #104
• transcriptome_fasta -> transcript_fasta by @leipzig in #97
• AWS test all profiles and fix publishdir by @ggabernet in #106
• fix full test by @ggabernet in #107
• add default to outdir as it was in schema by @fmalmeida in #108
• Fixes full tests with 10x references by @apeltzer in #109
• update CI and full size test by @ggabernet in #112
• Update nextflow.config by @fmalmeida in #111
• Fix for STAR chemistry issue #60 by @apeltzer in #113
• PR for Release 2.0.0 by @fmalmeida in #105

New Contributors

• @ggabernet made their first contribution in #74
• @grst made their first contribution in #77
• @medulka made their first contribution in #59
• @fmalmeida made their first contribution in #104
• @leipzig made their first contribution in #97

Full Changelog: 1.1.0…2.0.0

Several updates / fixes have been added, prior to converting the pipeline to DSLv2 and adding more advanced features.

• Template update with latest nf-core/tools v1.13.2
• Parameters JSON Schema added #42
• 25 Fix small documentation error with wrong parameter for txp2gene

Fixes

• #20 Fix Transcriptome Fasta argument not detected well
• #21 Fix --kallisto_index being ignored

v1.0.0 - 2019-12-09 “Tiny Aluminium Crab”

Initial release of nf-core/scrnaseq, created with the nf-core template. This includes the following workflow options:

• Salmon Alevin + AlevinQC
• STARSolo
• Kallisto / BUStools