nf-core/mcmicro
An end-to-end processing pipeline that transforms multi-channel whole-slide images into single-cell data.
bioformatsimage-analysisimage-processingmicroscopymultiplexed-imagingome-tiffregistrationsegmentationsingle-cellspatialstitchingtma
Version history
This release marks the point where the pipeline was moved from labsyspharm/mcmicro over to the new nf-core community, at nf-core/mcmicro.
View the previous changelog at labsyspharm/mcmicro/CHANGES.md
In addition to porting to the new nf-core community, the pipeline has had a number of major changes in this version.
- Input files can live anywhere not necessarily with output files.
- Input formatting is changed from a custom directory structure to a sample sheet see docs.
- No longer need for custom params.yml, now can be passed as command line options or as nextflows
-params-fileJSON format. - Added a
preludestep that verifies input image metadata and reports inconsistencies/errors. - Removed unmicst segmenter.
- Added cellpose as the new default segmenter, customized for smaller footprint!
- Basic illumination upgraded to the next generation
basicpytool.
And many performance quirks and bug fixes! We would like to thank everyone involved in this release!